【学术简介】

学习经历：

1994-1998年，南京大学生物化学系，学士

1998-2002年，香港科技大学生物化学系，博士研究生

教学及研究经历：

2002-2004年，香港科技大学生物化学系，研究员助理

2004-2009年，美国（Liver Diseases Branch, NIDDK, NIH）访问学者、博士后

2009-现在，江苏大学生命科学研究院，教授

学术社会兼职及荣誉：

中国微量元素协会、江苏毒理学会、中国致変剂学会

【主讲课程】

硕士研究生：生物化学，分子遗传

【指导硕、博士生研究方向】

博士/硕士研究生：金属代谢和毒理，蛋白质工程; Metabolism and toxicology of metals, Protein engineering

【研究领域】

本实验室是生命科学研究院主要定位于生物化学和生物医药方向的实验室，共有13位教学科研人员属于该方向与本实验室紧密合作。目前本实验室研究方向涉及三方面：1、重金属离子的毒理，镉干扰脂代谢的靶位点以及药物干预，镉的免疫毒理机制以及药物干预靶位点，镉致癌和癌转移的机制和药物干预靶位点；2、蛋白质工程，纳米酶工厂，改造酶以及医药蛋白增加稳定性和活性；3、于锋博士开展的细胞治疗技术。

My laboratory mainly focuses on the biochemistry and biomedical research, a total of 13 faculties associated with the research field in this institute. Currently, the research in this laboratory involves three aspects: 1, the toxicology of heavy metal ions, the molecular mechanism of cadmium disruption lipid metabolism and identification of the drug targets. The molecular mechanism of immunity impairment by cadmium and identification the drug target. Carcinogenesis and cancer metastasis mechanism of cadmium and identification of drug target; 2, Protein engineering, nano-enzyme factory, Modification of enzymes and pharmaceutical proteins to increase the stability and activity; 3. The cell therapy techniques are developing by Dr. Yu Feng.

【近5年论著和代表作】*通讯作者

1. Alex Tuffour, Anthony Adebayiga Kosiba, Yao Zhang, Frank Addai Peprah, Jie Gu, Haifeng Shi, Role of the calcium-sensing receptor (CaSR) in cancer metastasis to bone: Identifying a potential therapeutic target, Biochimica et Biophysica Acta (BBA) Reviews on Cancer, 1875 (2) 188528, April 2021, ISSN 0304-419X, https://doi.org/10.1016/j.bbcan.2021.188528. 二区

2. Shi H, Sun X, Kong A, Ma H, Xie Y, Cheng D, Wong CK, Zhou Y, Gu J Cadmium induces epithelial-mesenchymal transition and migration of renal cancer cells by increasing PGE2 through a cAMP/PKA-COX2 dependent mechanism Ecotoxicology and Environmental Safety 207 (2021) 111480 https://doi.org/10.1016/J.ECOENV.2020.111480一区

3. Peprah Addai F, Wang T, Kosiba AA, Lin F, Zhen R, Chen D, Gu J, Shi H*, Zhou Y*. Integration of elastin-like polypeptide fusion system into the expression and purification of Lactobacillus sp. B164 β-galactosidase for lactose hydrolysis Bioresource Technology 311, September 2020, 123513 一区

4. Gu J, Ren Z, Zhao J, Peprah FA, Xie Y, Cheng D, Wang Y, Liu H, Chu Wong CK, Zhou Y, Shi H*. Calcimimetic compound NPS R-467 protects against chronic cadmium induced mouse kidney injury by restoring autophagy process. Ecotoxicology and Environmental Safety 189 (2020) 110052 一区

5. Kosiba AA, Wang Y, Chen D, Wong CKC, Gu J, Shi H* The roles of calcium-sensing receptor (CaSR) in heavy metals-induced nephrotoxicity. Life Sci. 2020 Feb 1;242:117183. doi: 10.1016/j.lfs.2019.117183. Epub 2019 Dec 23.PMID: 31874167 Review 三区

6. Liu T, Gao Q, Yang B, Yin C, Chang J, Qian H, Xing G, Wang S, Li F, Zhang Y, Chen D, Cai J, Shi H, Aschner M, Appiah-Kubi K, He D, Lu R*. Differential susceptibility of PC12 and BRL cells and the regulatory role of HIF-1α signaling pathway in response to acute methylmercury exposure under normoxia. Toxicol Lett. 2020 May 24;331:82-91. doi: 10.1016/j.toxlet.2020.05.023. Epub ahead ofprint. PMID: 32461003. 二区

7. Xie Y, Yuan X, Zhou W, Kosiba AA, Shi H, Gu J, Qin Z. The circular RNA HIPK3 (circHIPK3) and its regulation in cancer progression: Review. Life Sci. 2020 Aug 1;254:117252. doi: 10.1016/j.lfs.2019.117252. Epub 2020 Jan 2. PMID: 31901445. 三区

8. Zhou Y, Li X, Yan D, Addai Peprah F, Ji X, Fletcher EE, Wang Y, Wang Y, Gu J, Lin F, Shi H*. Multifunctional elastin-like polypeptide renders β-glucosidase enzyme phase transition and high stability. Biotechnol Biofuels. 2019 Jun 24;12:157. doi: 10.1186/s13068-019-1497-5. eCollection 2019.PMID:31249620一区

9. Zhou Y, Yuan XD, Wang JC, Liu HT, Peprah FA, Jiang TY, Gu J, Shi HF* Molecular cloning, expression and characterization of secreted ferritin in the silkworm, Bombyx mori. Biometals. 2019 Oct;32(5):757-769. doi: 10.1007/s10534-019-00208-1. 2019 Jul 30.PMID:31363876 三区

10. Jie G, Wang Y, Liu Y, Shi M, Yin L, Hou Y, Zhou Y, Wong CKC, Chen D, Guo Z, Shi H* Inhibition of autophagy alleviates cadmium-induced mouse spleen and human B cells apoptosis Toxicol Sci. 2019 Apr 15. pii: kfz089. doi: 10.1093/toxsci/kfz089. 二区

11. Zhou Y, Wang Y, Li X, Peprah FA, Wang X, Liu H, Lin F, Gu J, Yu F, Shi H*. Applying microarray-based technique to study and analyze silkworm (Bombyx mori) transcriptomic response to long-term high iron diet. Genomics. 2019 Dec;111(6):1504-1513. doi: 10.1016/j.ygeno.2018.10.005. Epub 2018 Oct 31.PMID:30391296

12. Fletcher EE, Yan D, Kosiba AA, Zhou Y, Shi H*. Biotechnological applications of elastin-like polypeptides and the inverse transition cycle in the pharmaceutical industry. (Review) Protein Expr Purif. 2019 Jan;153:114-120. doi: 10.1016/j.pep.2018.09.006. Epub 2018 Sep 11. Review.PMID:30217600

13. Zhou Y, Yang G, Liu H, Chen Y, Li X, Ge J, Wang X, Niu H, Dong W, Jiang T, Shi H, Peng L, Liu L*. A Noninvasive and Donor-independent Method Simultaneously Monitors Rejection and Infection in Patients With Organ Transplant. Transplant Proc. 2019 Jul - Aug;51(6):1699-1705. doi: 10.1016/j.transproceed.2019.04.051.PMID:31399160

14. Wang Y, Ji X, Dai S, Liu H, Yan D, Zhou Y, Gu J, Shi H*. Cadmium induced redistribution of cholesterol by upregulating ABCA1 and downregulating OSBP. Journal of Inorganic Biochemistry (189) 199-207, 2018 二区

15. Gu J, Dai S, Liu Y, Liu H, Zhang Y, Ji X1, Yu F, Zhou Y, Chen L, Tse WKF, Wong CKC, Chen B, Shi H*. Activation of Ca²⁺-sensing receptor as a protective pathway to reduce Cadmium-induced cytotoxicity in renal proximal tubular cells. Scientific reports. 2018 Jan 18;8(1):1092. doi: 10.1038/s41598-018-19327-9. 三区

16. Ullah K, Addai Peprah F, Yu F, Shi H*. The application of prostate specific membrane antigen in CART-cell therapy for treatment of prostate carcinoma (Review) Oncol Rep. 4 3136 0: 3136-3143, 2018

17. Zhou Y, Yuan S, Liu Q, Yan D, Wang Y, Gao L, Han J, Shi H*. Synchronized purification and immobilization of his-tagged β-glucosidase via Fe3O4/PMG core/shell magnetic nanoparticles. Scientific reports. 2017 Jan 30;7:41741. doi: 10.1038/srep41741. 三区

18. Feng T, Liu H, Xu Q, Sun J, Shi H*. Identification and Characterization of Two Endogenous β-Glucosidases from the Termite Coptotermes formosanus. Appl Biochem Biotechno. 2015,176(7): 2039-2052. DOI: 10.1007/s12010-015-1699-7.  

19. He T, Liu H, Zhou Y, Yang J, Cheng X, Shi H*.Antibacterial effect and proteomic analysis of Graphene-based silver nanoparticles on a pathogenic bacteria Pseudomonas aeruginosa. Biometals, 2014, 27(4):673-682. DOI: 10.1007/s10534-014-9756-1三区

20. Shi H, Bencze K, Stemmler TL, and Philpott CC, Poly r(C)-Binding Protein 1 is a cytosolic iron chaperone that delivers iron to ferritin. Science, 2008, 30(5880):1207-10. DOI: 10.1126/science.1157643 一区

    【科研项目】

21. 2013.1 -2016.12，自然科学基金面上项目“通过蛋白组转录组和基因筛选研究镉诱导生物毒性的机理”， 31271272， 80万元，主持，完成

22. 2011.1 -2013.12，自然科学基金面上项目“人多聚胞嘧啶结合蛋白在铁代谢中的功能研究”， 31071030， 33万元，主持，完成

23. 2010年江苏省六大人才高峰，生物技术和新医药项目“人细胞铁代谢紊乱与人的相关疾病”

24. 2013年7月-2016年6月，江苏省自然科学基金青年基金项目“基于生物组学方法研究铁代谢调控家蚕免疫和生长的分子机制”BK20130506，25万，共同主持。完成

25. 2015年7月-2018年6月，江苏省自然科学基金面上项目“携带IL15和 sPD1的溶瘤痘病毒协同CAR-T抗癌及机制研究” BK20151347, 10万，参加，在研

26. 2015年6月-2017年12月，上海肿瘤所开放课题，“溶瘤痘病毒联合CAR-T靶向治疗肝癌研究”，10万，参加，在研

27. 2017.12-2020.12 游离DNA（cell-free DNA, cfDNA）在肾移植免疫排斥中的应用技术开发,  苏州人人基因科技有限公司,  200万，参加，在研

    【科研成果及奖励】

28. 2011年入选江苏省“333”工程第三层次

29. 2010年度江苏省双创项目“江苏省重点创新项目、重点学科、重点实验室、高层次引进人才”

30. 2012年度参与江苏省“新能源创新团队”

    【所获专利】

31. 一种C型β-葡萄糖苷酶突变体及其表达质粒和重组菌，201410801031.7，发明， 王继峰；吴黎明；冯婷婷；刘海涛；周阳；施海峰，2014-12-22，授权

32. 一种D型β-葡萄糖苷酶突变体及其表达质粒和重组菌，201410801030.2，发明，施海峰；冯婷婷；刘海涛；王继峰；吴黎明；周阳，2014-12-22，授权

33. 一种通过血清生物标志蛋白检测生物镉中毒的方法，201410470254.X，发明，施海峰;刘海涛；周阳；屠志刚；张修文；袁啸；史美琳，2014-09-15，授权

    【在读硕、博士人数】

    博士2人，硕士4人，留学生硕士1人

    【已毕业硕、博士人数】

    硕士数人