Curriculum Vitae

Name: Huayou Chen

Job  title: Professor. Supervisor of doctor and postdoctor. Director of institute of applied microbiology, jiangsu university (http:// iam.ujs.edu.cn). Director of department of Viruses and applied microbiology.
Phone number: 86-13912800258 (Wechat ID)

E-mail:   phdc@163.com

Educational qualifications:

12. 2013-12.2014, Postdoctor, Department of microbiology, University of California(SAN DIEGO)，America.

07.2009-12.2013, Postdoctor, Biological feed, Agricultural Engineering in Jiangsu University. China.

09.2003-07.2006, Ph.D, Molecular Microbiology in Shanghai Institutes for Biological Sciences (SIBS) of Chinese Academy of Sciences (CAS).

09.2000-07.2003, M.A., Biochemistry and Molecular Biology in East China Normal University

Job qualifications:

11.2007-Present. Professor in Jiangsu University

06.2007-11.2007, Research director in Shanghai Manrun Bio. Co., Ltd.

07.2006-06.2007, Researcher in Shanghai celstar Bio. Co., Ltd.

06.2003-09.2003, Microbiology researcher in Shanghai Siji Bio. Co., Ltd.

Fields of study:

1.Enzyme engineering and simulating computation 2.Bio-feed and genetic engineering 3.Biochemical engineering 4. Microbe metabolic engineering 5. Fermentation engineering. 6 Bacteria and algae interaction. 7.Aquaculture and Fermented Chinese herbal medicine. 8.Straw biological feed.

Research interest:

1 Creation of enzymes used for bulk unconventional feed resource

2   New pattern of feed protein and microbial feed, especially on straw biological feed.

3  Cell factory of Bacillus SP， Yeast，or lactic acid bacteria

(1) The surface display of recombinant enzyme on Bacillus subtilis spores

(2) Efficient food grade safety expression system of Bacillus subtilis. Yeast，or lactic acid bacteria

Major current research projects as principle investigator(PI):

1. National Key Research and Development Program of China (2021YFC2103004): “Creation of enzymes used for bulk unconventional feed resource”. 2021.12-2024.12. (5.1 million CNY)

2. National Key Research and Development Program of China (2021YFA0910402，): “Reconstruction of artificial electron transport chain module of glycometabolism”. 2021.12-2026.12. (0.94 million CNY)

3. Jiangsu Province Carbon Peak Carbon neutral Science and Technology Innovation project (Major agricultural project) : Integration of key technologies for efficient conversion of protein feed from agricultural waste carbon sources and industrialization. （BE2023399），2023.7-2027.6, Chief Scientist, (5 million CNY).

Major post research projects as principle investigator(PI):

1 “Cell factories of Bacillus sp ”. National Key Basic Research Program of China (973 Program) NO. 2011CBA00801.  01/2011.1-12/2015. (one million CNY).

2 “key technology of monogastric animal Bio-feed”. The Key Research Project(Modern Agriculture) of Jiangsu Province, China (No. BE2017355), 2017.7.1-2020.12. (0.5 million CNY).

3 “key technology of Bio-feed from agricultural wastes”. Jiangsu Agriculture Science and Technology Innovation Fund （JASTIF，no. CX(17)3044)，2017.7.1-2020.12. (0.3 million CNY).

4 “Straw biological feed”.The Key Agriculture Support Project of Jiangsu Province, China (No. BE2013400), 2013.7-2016.12, (0.5 million CNY)

5 “Influence mechanism of the linker to the thermophilic lipase displayed on the Surfaces of Bacillus subtilis Spores”. The Open Funding Project of the State Key Laboratory of Biochemical Engineering, China.(2018KF-02). 2018.1-2021.12. (0.1 million CNY).

6 “Research of metabolic engineering of the thermophilic anaerobic bacteria and the cellulase”, Sub-project of National Key Basic Research Program of China (973 Program) NO. 2009CB724700. 09/2009-12/2013. (0.2 million CNY).

7 “ Development and industrialization of microbial agent against coccidiosis in chicken”. Collaboration Project in the Changjiang River Delt, 05/2011.-05/2013. (0.6 million CNY).

8 “Key technology of Bio-feed from Fibrous brew dregs”. The Open Funding Project of the State Key Laboratory of Biochemical Engineering, China.(2013KF-02). 2013.1-2015.12. (0.15 million CNY).

Selected publications:

55. Huayou Chen,* Lingyu Kang, Keyi Li, Tao Feng, Zhong Ni, Ebin Gao & Zhen Fang. Accurate quantification on the change of some live pathogenic microbial flora in fermented feed.  International Journal of Food Science and Technology，2023.5. Q1

54. Zhen Wang, Mingchen Yan, Keyi Li, Yanzhen Chen, Zhen Fang, Zhong Ni, Huayou Chen *. The flexible linker and CotG were more effective for the spore surface display of keratinase KERQ7. World Journal of Microbiology and Biotechnology.2023.12. Q2

53. Mati Ullah , Muhammad Rizwan, Ali Raza, Yutong Xia, Jianda Han, YiMa , Huayou Chen*. Snapshot of the Probiotic Potential of Kluveromyces marxianus DMKU-1042 Using a Comparative Probiogenomics Approach.  Foods.2023.12 Q1

52. Shouzhi Li, Lu He, Na Shi, and Huayou Chen*.  Display and identification of lignin peroxidase on the surface of Bacillus subtilis", Applied Biochemistry and Biotechnology.2023.12. Q2

51. Keyi Li , Yutong Xia, Zhen Wang, Ebin Gao, Shuhao Huo, Huayou Chen*. Progress in the cultivation of diatoms using organic carbon sources. Algal Research. 74 (Jul. 2023) 103191. IF5.6, Q1

50. Zhen Wang1 · Yanzhen Chen1 · Mingchen Yan1 · Keyi Li1 · Charles Obinwanne Okoye2,3 · Zhen Fang2 · Zhong Ni1 .Huayou Chen*.  Research progress on the degradation mechanism and modification of keratinase. Applied Microbiology and Biotechnology (Feb. 2023) 107:1003–1017. （中科院2区IF5.59,  Q1）

49 Jibin Li , Xinyu Heng, Zhong Ni and Huayou Chen *. Study on the Antibacterial Rule in Fermented Feed with Different Amounts of CaCO3 by Quantitative Real-Time Polymerase Chain Reaction. Fermentation 2023, 9(11), 940. 中科院2区

48 Muhammad Saeed，Nazar Hussaina,and Huayou Chen*. The potential of lactoferrin: a call for future research in poultry nutrition. World's Poultry Science Journal. Aug 2023. Q1

47. Muhammad Saeed，and Huayou Chen*.Nutritional Significance and Health Benefits of Quail’s Meat and Eggs: An Empirical Review. Pakistan Journal of Agricultural Sciences，2023

46 Shouzhi Li, Lu He, Na Shi, Yanzhen Chen, Muhammad Saeed, Zhong Ni, and Huayou Chen*.  Preparing the pure lignin peroxidase and exploring the effects of chemicals on the activity. Preparative Biochemistry & Biotechnology. Oct. 2023.

45. Muhammad Saeed，and Huayou Chen*. Food Free of Antibiotic Residues: Use of Postbiotic as Growth Promoter in Poultry Industry: A Review of Current Knowledge and Future Prospects. Food Science of Animal Resources, 2023.11  Q1

44. Huayou Chen* · Xiaoyu Liang · Shouzhi Li ·Zhoulei Cui · Yangchun Yong · Zhong Ni ·Quan Bu · Daochen Zhu. Straw lignin degradation by lignin peroxidase from Irpex lacteus cooperated with enzymes and small molecules. Biotechnol Lett.2023.1.

43. Huayou Chen*, Xinyu Heng, Keyi Li, Zhen Wang, Zhong Ni, Ebin Gao, Yangchun Yong and Xin Wu*. Complexation of multiple mineral elements by fermentation and its application in laying hens. Frontiers in Nutrition. 2022.8.29（IF6.59, 中科院1区JCR1）

42. Xinyu Heng, Huayou Chen *, Chenxi Lu, Tao Feng, Keyi Li, Ebin Gao . Study on synergistic fermentation of bean dregs and soybean meal by multiple strains and proteases. LWT - Food Science and Technology, Volume 154, 15 January 2022, 112626. （IF6.0, 中科院1区JCR1）

41. Heng, XY ; Chen, HY* ; Li, JB. Study on the removal of anti-nutritional factors in bean dregs by solid-state fermentation.Pakistan Journal of Agricultural Sciences （Pak. J. Agri. Sci.）, Vol. 59(4), 543-551; 2022.8

40.Huayou Chen*, Shouzhi Li, Zhoulei Cui, Tao Feng, Hongcheng Wang, Zhong Ni, Ebin Gao & Zhen Fang. Synergistic Degradation of Maize Straw Lignin by Manganese Peroxidase from Irpex lacteus. Applied Biochemistry and Biotechnology. 17 October 2022.

39. Huayou Chen*, Chenxi Lu, Tingting Li, Lingyu Kang, Xiaoyu Liang, Zhong Ni. Feasibility study of fluorescence quantitative PCR for the detection of microecological dynamics in fermented maize stover feeds. Rom Biotechnol Lett. 2021; 26(5): 2986-2993.

38. Huayou Chen*, Xiaoyu Liang, Kangtao Cai, Bangguo Wu, Hongcheng Wang , Zhong Ni. Detoxification of corn stalk pretreated with calcium hydroxide for true protein accumulation. Rom Biotechnol Lett. 2021; 26(6): 3137-3144

37. Huayou Chen*, Kangtao Cai, Lingyu Kang,Tingting Li, Xiaoyu Liang, Xinyu Heng

Identification of Lactic Acid Bacteria in the corn stalk Fermentation by Fluorescence Quantitative PCR. Rom Biotechnol Lett. 2021; 26(5): 2926-2935

36. Kangtao Cai, Huayou Chen*, Xinyu Heng, Lingyu Kang,Junming Wu, Chenxi Lu, Xiaoyu Liang. Optimization of Small Peptide Feed from Milk Thistle Residue by

Synergistic Fermentation of Multiple Strains and Proteases. Rom Biotechnol Lett. 2021; 26(6): 3102-3109

35. Xinyu Heng, Huayou Chen*, Jibin Li, Kangtao Cai, Chenxi Lu. Optimization of Fermentation Process for Improving Soy Isoflavones Aglycone Content in Bean Dregs by Lactobacillus plantarum PL70a. Rom Biotechnol Lett. 2021; 26(5): 2942-2952

34. KailiMao, YaoZhu, JianRong, FengxianQiu*, HuayouChen*, JinchaoXu, DongyaYang, TaoZhang, LiZhong. Rugby-ball like Ag modified zirconium porphyrin metal–organic frameworks nanohybrid for antimicrobial activity: Synergistic effect for significantly enhancing photoactivation capacity. Colloids and Surfaces A: Physicochemical and Engineering Aspects. Volume 611, 20 February 2021, 125888.

33. Jinru Jia, Huayou Chen* , Bangguo Wu,Fengjie Cui, Hua Fang, Hongcheng Wang, Zhong Ni. Protein Production Through Microbial Conversion of Rice Straw by Multi-Strain Fermentation . Applied Biochemistry and Biotechnology, 2019 Jan;187(1):253-265. (SCI)

32. Huayou Chen*, Bangguo Wu, Tianxi Zhang, Jinru Jia, Jian Lu, Zhi Chen, Zhong Ni,Tianwei Tan.  Effect of Linker Length and Flexibility on the Clostridium thermocellum Esterase Displayed on Bacillus subtilis Spores. Applied Biochemistry and Biotechnology. 2017, 182(1):168–180. (SCI)

31. Huayou Chen*, Jinru Jia, Zhong Ni, Ake Vastermark, Bangguo Wu, Yilin Le and Ullah Jawad. Orlistat response to missense mutations in lipoprotein lipase. Biotechnology and Applied Biochemistry. 2017,64(4):464-470. (SCI)

30. Huayou Chen*, Zhi Chen, Bangguo Wu, Jawad Ullah, Tianxi Zhang, Jinru Jia, Hongcheng Wang , Tianwei Tan. Influences of various peptide linkers on the Thermotoga maritima MSB8 nitrilase displayed on the spore surface of B. subtilis.Journal of Molecular Microbiology and Biotechnology. 2017;27(1):64-71. (SCI)

29. Huayou Chen* , Jawad Ullah, Jinru Jia. Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis,  J Mol Microbiol Biotechnol 2017;27:159–167(SCI)

28. Jawad Ullah, Huayou Chen*, Ake Vastermark, Jinru Jia, Bangguo Wu, Zhong Ni, Yilin Le, Hongcheng Wang.  Impact of Orientation and flexibility of peptide linkers on T. Maritima lipase Tm1350 displayed on Bacillus Subtilis spores surface using CotB as fusion partner. World Journal of Microbiology and Biotechnology. 2017. 9, 33:166(SCI)

27. Jinru Jia, Huayou Chen*, Bangguo Wu, Zhong Ni. Cations Optimization for Protein Enrichment in Rice Straw by Mixed Cultures of Neurospora crassa 14-8 and Candida utilis Using Response Surface Methodology. Applied Biochemistry and Biotechnology. 2017, 182(2):804-817. (SCI)

26. Huayou Chen*, Zhi Chen, Zhong Ni, Rui Tian, Tianxi Zhang, Jinru Jia, Keping Chen, Shengli Yang. Display of Thermotoga maritima MSB8 nitrilase on the spore surface of Bacillus Subtilis using out coat protein CotG as the fusion partner. Journal of Molecular Catalysis B: Enzymatic. Jan. 2016, 123: 73–80 (SCI)

25. Chen H*.· Zhang T. · Sun T. · Ni Z. · Le Y. · Tian R. · Chen Z. · Zhang C. Clostridium thermocellum Nitrilase Expression and Surface Display on Bacillus subtilis Spores. Journal of Molecular Microbiology and Biotechnology. 2015;25:381-387 (SCI).

24.Huayou Chen*, Rui Tian, Zhong Ni, Qing Zhang, Tianxi Zhang, Zhi Chen, Keping Chen, Shengli Yang. Surface display of the thermophilic lipase Tm1350 on the spore of Bacillus subtilis by the CotB anchor protein. Extremophiles. July 2015, 19(4), pp 799-808 (SCI).

23. Huayou Chen*, Tianxi Zhang, Jinru Jia, Ake Vastermark, Rui Tian, Zhong Ni, Zhi Chen, Keping Chen, Shengli Yang.Expression and display of a novel thermostable esterase from Clostridium thermocellum on the surface of Bacillus subtilis using the CotB anchor protein.Journal of Industrial Microbiology & Biotechnology.Nov.2015, 42(11), pp 1439-1448 (SCI).

22.Rui Tian,Huayou Chen*, Zhong Ni, Qing Zhang, Zhongge Zhang, Tianxi Zhang, Chunxia Zhang, Shengli Yang.Expression and Characterization of a Novel Thermo-Alkalistable Lipase from Hyperthermophilic Bacterium Thermotoga maritima.Applied Biochemistry and Biotechnology. July 2015, 176(5), pp 1482-1497. (SCI).

21.Tianxi Zhang,Huayou Chen*,Zhong Ni, Rui Tian,Jinru Jia, Zhi Chen,Shengli Yang.Expression and characterization of a new thermostable esterase from Clostridium thermocellum.Applied Biochemistry and Biotechnology.2015,177: 1437-1446.(SCI).

20.Zhi Chen,Huayou Chen*, Zhong Ni, Rui Tian, Tianxi Zhang, Jinru Jia, Shengli Yang.Expression and characterization of a novel nitrilase from hyperthermophilic bacterium Thermotoga maritima MSB8. JMB. 2015,25(10): 1660-1669.(SCI).

19.Huayou Chen, Rui Tian, Zhong Ni, Zhongge Zhang, Hongzhang Chen, Qi Guo, Milton H Saier. Conformational Transition Pathway in the Inhibitor Binding Process of Human Monoacylglycerol Lipase. Protein J (2014) 33:503–511.(SCI).

18.Huayou ChenTengyun Sun Zhong Ni.Structural and energetic insights into the selective interactions of monoacylglycerol lipase with its natural substrate and small-molecule inhibitors.

Medicinal Chemistry Research. May 2014, 23(5), pp 2391-2404 (SCI).

17.Rongzhong Jin • Yili Ma •Huayou Chen*.Structural and Affinity Insight into the Sequence-Specific Interaction of Transcription Factors DEC1 and DEC2 with E-box DNA: A Novel Model Peptide Approach. Int J Pept Res Ther. 19 June 2013. DOI 10.1007/s10989-013-9354-z. (SCI)

16.Huayou Chen,Zhongmei Chu, Yi Zhang, Shengli Yang.Overexpression and Characterization of the recombinant Small Heat Shock Protein fromPyrococcus furiosus.Biotechnology Letters.2006,28(14):1089-1094. (SCI)

15.Hua-You Chen, Zhong-Mei Chu, Yan-he ma,Yi Zhang , Sheng-Li Yang. Expression and Characterization of the Chaperonin Molecular Machine from the Hyperthermophilic ArchaeonPyrococcus furiosus, Journal of Basic Microbiology2007,47(12):132 – 137. (SCI)

14. Hua-you Chen, Xiao-li Tan, Jian Lu, Chun-xia Zhang,Yi Zhang, Sheng-liYang.Characterization of the ATPase activity of class II chaperonin from the hyperthermophilic archaeonPyrococcus furiosus.Biotechnology Letters. 2009, 31:1753–1758 (SCI)

13. CHEN Hua-you,QI Xiang-hui, GENG Xu, XU Qing-gang. Separation and Purification of Recombinant Hirudin Variant 3 fromBacillus subtilis.Agricultural Science and Technology, 2009, 10(6) p.15-19.

12. Hua-you Chen,Jin-hong Zhang, Jian Lu, Qing-gang Xu, Xiang-hui Qi, Dao-chen Zhu. Simple and efficient methods for isolation and activity measurement of the recombinant hirudin variant 3 from the Bacillus subtilis. African Journal of Biotechnology. 2011,10(44)8889-8894. (SCI)

11. Huayou Chen, Xianghui Qi, Xu Geng, Qinggang Xu, Jing Wang, Zirong Wu.Expression, Purification and Characterization of the Recombinant Hirudin Variant iii in the Bacillus Subtilis.Advanced Materials ResearchVols. 343-344 (2012) pp 753-763. (EI )

10.YILIN Le,HUAYOU Chen, ROBERT Zagursky, J.H. DAVID Wu,*, and WEILAN Shao,*.Thermostable DNA Ligase-Mediated PCR Production of Circular Plasmid (PPCP) and Its Application in Directed Evolution via In situ Error-Prone PCRDNA RESEARCH 20, 375–382, (2013). (SCI，IF5)

9. Zhong Ni, Rongzhong Jin,Huayou Chen, Xianfu Lin. Just an additional hydrogen bond can dramatically reduce the catalytic activity of B. sub lipase A I12T mutant: an integration of computational modeling and experimental analysis. Computers in Biology and Medicine 2013, 43: 1882-1888. (SCI)

8. Zhong Ni,Huayou Chen, et al. Insight into molecular mechanism underlying the transesterification catalysed by penicillin G amidase(PGA) using a combination protocol of experimental assay and theoretical analysis. Molecular Simulation,2014, 40:14, 1125-1130, (SCI)

7. Zhong Ni,Huayou Chen, Xianghui Qi. Why is substrate peptide binding unsusceptible to multidrug-resistant mutations in HIV-1 protease? A structural and energetic analysis. Int. J. Pept. Res. Ther.2014 (online, DOI: 10.1007/s10989-013-9365-9). (SCI)

6. Huayou Chen *, Shengli Yang. Sec-Dependent Protein*Jinhong Zhang, Pingping Li , Degang Ning. Secretion Pathway of Bacillus Subtilis. 2011 Inernational Symposium on Biomedicine and Engineering.2011,pp570-575. (ISTP)

5.  Huayou Chen, Jinhong Zhang, Jian Lu , Pingping Li, Shengli Yang.Consolidated bioprocessing of biomass to ethanol by thermophilic anaerobic bacteria.2011 Inernational Symposium on Biomedicine and Engineering.2011,pp576-581. (ISTP)

4. Jinhong Zhang,Huayou Chen*, Pingping Li. Research Progress of Bacteria Cellulase，2011 International Conference on Environmental Systems Science and Engineering.2011, PP168-175.(ISTP).

3. Xiangfei Li, Wei Pan, Fengjie Cui, Yuguang Zhou,Huayou Chen*. Effect of Sludge from Different Sources on Solid Anaerobic Digestion of Gorn Straw. 2012,13(3):605-607.

2. Jian Lu,Huayou Chen; Qinggang Xu; Jianzhou Zheng; Haijun Liu;Jun Li; Keping Chen .ComparativeProteomicsof Kidney Samples from Puffer Fish Takifugu rubripes Exposed to Excessive Fluoride: An Insight into Molecular Response to Fluorosis. Toxicology Mechanisms and Methods, 2010, 20(6):345-54.（SCI）

1. Lisa Wang, Qinhua Zhou,Huayou Chen,Zhongmei Chu, Jianfeng Lu, Yi Zhang and Shengli Yang. Efficient solubilization, purification of recombinant extracellular α-amylase from pyrococcus furiosus expressed as inclusion bodies in Escherichia coli.Journal of Industrial Microbiology and Biotechnology.2007 Mar; 34(3):187-92.(SCI)

Patent for invention:

34 patents till now.