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April 17, 2020
Chen Huayou

Curriculum Vitae



Name: Huayou Chen

Job  title: Professor. Director of department of Viruses and applied microbiology. Director of institute of applied microbiology, jiangsu university (http:// iam.ujs.edu.cn)
Phone number: 86-13912800258 (Wechat ID)

Fax   number: 86-0511-88791923
E-mail:   phdc@163.com
Mailing address: Institute of life science, JiangsuUniversity 80#, Xuefu Road 301, Zhenjiang, Jiangsu, P.R.China, 212013

Educational qualifications:

12.2013-12.2014, Postdoctor, Department of microbiology, University of California(SAN DIEGO)

07.2009-12.2013, Postdoctor, Biological feed, Agricultural Engineering in Jiangsu University

09.2003-07.2006, Ph.D, Molecular Microbiology in Shanghai Institutes for Biological Sciences (SIBS) of Chinese Academy of Sciences (CAS).

09.2000-07.2003, M.A., Biochemistry and Molecular Biology in East China Normal University

 Job qualifications:

11.2007-Present. Professor in Jiangsu University

06.2007-11.2007, Research director in Shanghai Manrun Bio. Co., Ltd.

07.2006-06.2007, Researcher in Shanghai celstar Bio. Co., Ltd.

06.2003-09.2003, Microbiology researcher in Shanghai Siji Bio. Co., Ltd.

Fields of study:

1.Biochemical engineering  2.Bio-feed and genetic engineering  3.Enzyme engineering  4. Microbe metabolic engineering  5. Fermentation engineering  

Research interest:

1  Cell factory of Bacillus SP,Yeastor lactic acid bacteria

(1) The surface display of recombinant enzyme on Bacillus subtilis spores

(2) Efficient food grade safety expression system of Bacillus subtilis. Yeastor lactic acid bacteria

2   New pattern of feed protein and microbial feed

Major current research projects as principle investigator(PI):

1 “key technology of monogastric animal Biofeed”. The Key Research Project(Modern Agriculture) of Jiangsu Province, China (no. BE2017355), 2017.7.1-2020.12. (0.5 million yuan).

2 “key technology of Biofeed from agricultural wastes”. Jiangsu Agriculture Science and Technology Innovation Fund JASTIFno. CX(17)3044)2017.7.1-2020.12. (0.3 million yuan).

3 “Influence mechanism of the linker to the thermophilic lipase displayed on the Surfaces of Bacillus subtilis Spores”. The Open Funding Project of the State Key Laboratory of Biochemical Engineering, China.(2018KF-02). 2018.1-2021.12. (0.1 million yuan).

Major post research projects as principle investigator(PI):

1 “Cell factories of Bacillus sp ”. National Key Basic Research Program of China (973 Program) NO. 2011CBA00801.  01/2011.1-12/2015. (one million yuan).

2 “Research of metabolic engineering of the thermophilic anaerobic bacteria and the cellulase”, Sub-project of National Key Basic Research Program of China (973 Program) NO. 2009CB724700. 09/2009-12/2013. (0.2 million yuan).

3 “Straw biological feed”.The Key Agriculture Support Project of Jiangsu Province, China (No. BE2013400), 2013.7-2016.12, (0.5 million yuan)

4 “ Development and industrialization of microbial agent against coccidiosis in chicken”. Collaboration Project in the ChangjiangRiver Delt, 05/2011.-05/2013. (0.6 million yuan).

5 Key technology of Biofeed from Fibrous brew dregs”. The Open Funding Project of the State Key Laboratory of Biochemical Engineering, China.(2013KF-02). 2013.1-2015.12. (0.15 million yuan).

Selected publications:

0, Jinru Jia, Huayou Chen* , Bangguo Wu,Fengjie Cui, Hua Fang, Hongcheng Wang, Zhong Ni. Protein Production Through Microbial Conversion of Rice Straw by Multi-Strain Fermentation . Applied Biochemistry and Biotechnology, 2019 Jan;187(1):253-265. (SCI)

1. Huayou Chen*, Bangguo Wu, Tianxi Zhang, Jinru Jia, Jian Lu, Zhi Chen, Zhong Ni,Tianwei Tan.  Effect of Linker Length and Flexibility on the Clostridium thermocellum Esterase Displayed on Bacillus subtilis Spores. Applied Biochemistry and Biotechnology. 2017, 182(1):168–180. (SCI)

2. Huayou Chen*, Jinru Jia, Zhong Ni, Ake Vastermark, Bangguo Wu, Yilin Le and Ullah Jawad. Orlistat response to missense mutations in lipoprotein lipase. Biotechnology and Applied Biochemistry. 2017,64(4):464-470. (SCI)

3. Huayou Chen*, Zhi Chen, Bangguo Wu, Jawad Ullah, Tianxi Zhang, Jinru Jia, Hongcheng Wang , Tianwei Tan. Influences of various peptide linkers on the Thermotoga maritima MSB8 nitrilase displayed on the spore surface of B. subtilis.Journal of Molecular Microbiology and Biotechnology. 2017;27(1):64-71. (SCI)

4. Huayou Chen* , Jawad Ullah, Jinru Jia. Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis,  J Mol Microbiol Biotechnol 2017;27:159–167(SCI)

5. Jawad Ullah, Huayou Chen*, Ake Vastermark, Jinru Jia, Bangguo Wu, Zhong Ni, Yilin Le, Hongcheng Wang.  Impact of Orientation and flexibility of peptide linkers on T. Maritima lipase Tm1350 displayed on Bacillus Subtilis spores surface using CotB as fusion partner. World Journal of Microbiology and Biotechnology. 2017. 9, 33:166(SCI)

6. Jinru Jia, Huayou Chen*, Bangguo Wu, Zhong Ni. Cations Optimization for Protein Enrichment in Rice Straw by Mixed Cultures of Neurospora crassa 14-8 and Candida utilis Using Response Surface Methodology. Applied Biochemistry and Biotechnology. 2017, 182(2):804-817. (SCI)

7. Huayou Chen*, Zhi Chen, Zhong Ni, Rui Tian, Tianxi Zhang, Jinru Jia, Keping Chen, Shengli Yang. Display of Thermotoga maritima MSB8 nitrilase on the spore surface of Bacillus Subtilis using out coat protein CotG as the fusion partner. Journal of Molecular Catalysis B: Enzymatic. Jan. 2016, 123: 73–80 (SCI)

8. Chen H*.· Zhang T. · Sun T. · Ni Z. · Le Y. · Tian R. · Chen Z. · Zhang C. Clostridium thermocellum Nitrilase Expression and Surface Display on Bacillus subtilis Spores. Journal of Molecular Microbiology and Biotechnology. 2015;25:381-387 (SCI).

9.Huayou Chen*, Rui Tian, Zhong Ni, Qing Zhang, Tianxi Zhang, Zhi Chen, Keping Chen, Shengli Yang. Surface display of the thermophilic lipase Tm1350 on the spore of Bacillus subtilis by the CotB anchor protein. Extremophiles. July 2015, 19(4), pp 799-808 (SCI).

10. Huayou Chen*, Tianxi Zhang, Jinru Jia, Ake Vastermark, Rui Tian, Zhong Ni, Zhi Chen, Keping Chen, Shengli Yang.Expression and display of a novel thermostable esterase from Clostridium thermocellum on the surface of Bacillus subtilis using the CotB anchor protein.Journal of Industrial Microbiology & Biotechnology.Nov.2015, 42(11), pp 1439-1448 (SCI).

11.Rui Tian,Huayou Chen*, Zhong Ni, Qing Zhang, Zhongge Zhang, Tianxi Zhang, Chunxia Zhang, Shengli Yang.Expression and Characterization of a Novel Thermo-Alkalistable Lipase from Hyperthermophilic Bacterium Thermotoga maritima.Applied Biochemistry and Biotechnology. July 2015, 176(5), pp 1482-1497. (SCI).

12.Tianxi Zhang,Huayou Chen*,Zhong Ni, Rui Tian,Jinru Jia, Zhi Chen,Shengli Yang.Expression and characterization of a new thermostable esterase from Clostridium thermocellum.Applied Biochemistry and Biotechnology.2015,177: 1437-1446.(SCI).

13.Zhi Chen,Huayou Chen*, Zhong Ni, Rui Tian, Tianxi Zhang, Jinru Jia, Shengli Yang.Expression and characterization of a novel nitrilase from hyperthermophilic bacterium Thermotoga maritima MSB8. JMB. 2015,25(10): 1660-1669.(SCI).

14.Huayou Chen, Rui Tian, Zhong Ni, Zhongge Zhang, Hongzhang Chen, Qi Guo, Milton H Saier. Conformational Transition Pathway in the Inhibitor Binding Process of Human Monoacylglycerol Lipase. Protein J (2014) 33:503–511.(SCI).

15.Huayou ChenTengyun Sun Zhong Ni.Structural and energetic insights into the selective interactions of monoacylglycerol lipase with its natural substrate and small-molecule inhibitors.

Medicinal Chemistry Research. May 2014, 23(5), pp 2391-2404 (SCI).

16.Rongzhong Jin • Yili Ma •Huayou Chen*.Structural and Affinity Insight into the Sequence-Specific Interaction of Transcription Factors DEC1 and DEC2 with E-box DNA: A Novel Model Peptide Approach. Int J Pept Res Ther. 19 June 2013. DOI 10.1007/s10989-013-9354-z. (SCI)

17.Huayou Chen,Zhongmei Chu, Yi Zhang, Shengli Yang.Overexpression and Characterization of the recombinant Small Heat Shock Protein fromPyrococcus furiosus.Biotechnology Letters.2006,28(14):1089-1094. (SCI)

18.Hua-You Chen, Zhong-Mei Chu, Yan-he ma,Yi Zhang , Sheng-Li Yang. Expression and Characterization of the Chaperonin Molecular Machine from the Hyperthermophilic ArchaeonPyrococcus furiosus, Journal of Basic Microbiology2007,47(12):132 – 137. (SCI)

19. Hua-you Chen, Xiao-li Tan, Jian Lu, Chun-xia Zhang,Yi Zhang, Sheng-liYang.Characterization of the ATPase activity of class II chaperonin from the hyperthermophilic archaeonPyrococcus furiosus.Biotechnology Letters. 2009, 31:1753–1758 (SCI)

20. CHEN Hua-you,QI Xiang-hui, GENG Xu, XU Qing-gang. Separation and Purification of Recombinant Hirudin Variant 3 fromBacillus subtilis.Agricultural Science and Technology, 2009, 10(6) p.15-19.

21. Hua-you Chen,Jin-hong Zhang, Jian Lu, Qing-gang Xu, Xiang-hui Qi, Dao-chen Zhu. Simple and efficient methods for isolation and activity measurement of the recombinant hirudin variant 3 from the Bacillus subtilis. African Journal of Biotechnology. 2011,10(44)8889-8894. (SCI)

22. Huayou Chen, Xianghui Qi, Xu Geng, Qinggang Xu, Jing Wang, Zirong Wu.Expression, Purification and Characterization of the Recombinant Hirudin Variant iii in the Bacillus Subtilis.Advanced Materials ResearchVols. 343-344 (2012) pp 753-763. (EI )

23.YILIN Le,HUAYOU Chen, ROBERT Zagursky, J.H. DAVID Wu,*, and WEILAN Shao,*.Thermostable DNA Ligase-Mediated PCR Production of Circular Plasmid (PPCP) and Its Application in Directed Evolution via In situ Error-Prone PCRDNA RESEARCH 20, 375–382, (2013). (SCIIF5)

24. Zhong Ni, Rongzhong Jin,Huayou Chen, Xianfu Lin. Just an additional hydrogen bond can dramatically reduce the catalytic activity of B. sub lipase A I12T mutant: an integration of computational modeling and experimental analysis. Computers in Biology and Medicine 2013, 43: 1882-1888. (SCI)

25.Zhong Ni,Huayou Chen, et al. Insight into molecular mechanism underlying the transesterification catalysed by penicillin G amidase(PGA) using a combination protocol of experimental assay and theoretical analysis. Molecular Simulation,2014, 40:14, 1125-1130, (SCI)

26.Zhong Ni,Huayou Chen, Xianghui Qi. Why is substrate peptide binding unsusceptible to multidrug-resistant mutations in HIV-1 protease? A structural and energetic analysis. Int. J. Pept. Res. Ther.2014 (online, DOI: 10.1007/s10989-013-9365-9). (SCI)

27. Huayou Chen *, Shengli Yang. Sec-Dependent Protein*Jinhong Zhang, Pingping Li , Degang Ning. Secretion Pathway of Bacillus Subtilis. 2011 Inernational Symposium on Biomedicine and Engineering.2011,pp570-575. (ISTP)

28. Huayou Chen, Jinhong Zhang, Jian Lu , Pingping Li, Shengli Yang.Consolidated bioprocessing of biomass to ethanol by thermophilic anaerobic bacteria.2011 Inernational Symposium on Biomedicine and Engineering.2011,pp576-581. (ISTP)

29.Jinhong Zhang,Huayou Chen*, Pingping Li. Research Progress of Bacteria Cellulase2011 International Conference on Environmental Systems Science and Engineering.2011, PP168-175.(ISTP).

30.Xiangfei Li, Wei Pan, Fengjie Cui, Yuguang Zhou,Huayou Chen*. Effect of Sludge from Different Sources on Solid Anaerobic Digestion of Gorn Straw. 2012,13(3):605-607.

31.Jian Lu,Huayou Chen; Qinggang Xu; Jianzhou Zheng; Haijun Liu;Jun Li; Keping Chen .ComparativeProteomicsof Kidney Samples from Puffer Fish Takifugu rubripes Exposed to Excessive Fluoride: An Insight into Molecular Response to Fluorosis. Toxicology Mechanisms and Methods, 2010, 20(6):345-54.SCI

32.Lisa Wang, Qinhua Zhou,Huayou Chen,Zhongmei Chu, Jianfeng Lu, Yi Zhang and Shengli Yang. Efficient solubilization, purification of recombinant extracellular α-amylase from pyrococcus furiosus expressed as inclusion bodies in Escherichia coli.Journal of Industrial Microbiology and Biotechnology.2007 Mar; 34(3):187-92.(SCI)

Patent for invention:

20 patents till now.


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