Curriculum Vitae
Name: Weijun Mai
Education: Doctor
Academic title: Associate Professor, Masters tutor
Postal Code: 212013
Subject categories: Molecular biology
Mailing Address: 301 Xuefu Road,Zhenjiang，China
E-mail: mwj9876@yahoo.com.cn
Educational Background
Ph. D., South China Sea Institute of Oceanology, CAS, 2005
M.S., South China Institute of Botany, 2001
B.S., Guangzhou University, 1997
Professional Experience
assistant research fellow, The Institute of Life Science, Jiangsu University
2010 –
Postdoctor, School Of Life Science, South China Normal University,
2008– 2010
Research Works
My research interests include molecular cell biology and molecular immunity of the model organisms. The current research focuses on PPARs functions and role on cancer cell proliferation, mentabolism, autophagy, and immuno escape.
Research experiences
During the last 10 years I was interested in the fields of marine biology, molecular biology and immunity.
Past research and achievements were described as follow:
1. As an assistant research fellow in The Institute of Life Science, Jiangsu University, I am studying the function of some fish immume response genes. My study includes (Ⅰ) function of transcription factors e.g. a tilapia p53 gene in the DNA damage and repair induced by stresses. (One paper has been accepted by Biotechnology letters; (Ⅱ): mRNAs-Wide Identification, Classification, and Expression Analysis of immune response genes in head kidney and peripheral blood of red-spotted grouper induced by
Vibrio alginolyticus. (one paper has been preparing to submit to Fish and shellfish immunology and acquired the support from Chinese National Science Foundation (30900116)); (Ⅲ): foreign gene expression and protein production in silkworm bioreactor; DNA replication, repair, and cell cycle regulation (one paper has been accepted by Plos One).
2. As a postdoctor in South China Normal University, advised by Prof. Weina Wang, I studied protection of shrimp against white spot syndrome virus (WSSV) challenge by lysozyme and role of ATM-p53 pathway in the DNA damage response when responding to acute acid stress. I found that lysozyme is effective at blocking WSSV infection in
L. stylirostris and that lysozyme modulates the cellular and humoral defense mechanisms after they are suppressed by the WSSV virus. I have used a genetic approach to dissect components of the p53 pathway that contribute to DNA repair responses caused by acute acid stress. The observation of the studies suggested that acute acid stress activates the protein kinase activities of ATM and downstream p53 that cause moderate genotoxic damage in blood cells of tilapias. In particular, p21 contributes appreciably to DNA repair, while GADD45 contributes little. One interpretation is that p53-associated repair may be dependent of the G1 cell cycle checkpoint mediated by p21. The results also demonstrate p53 regulation of genes encoding antioxidant enzymes such as MnSOD and GPx to lighten cellular damage by acute acid stress.
3. As a doctoral student in South China Sea Institute of Oceanology, Chinese Academy of Sciences, advised by Prof. Chaoqun Hu, I studied the genetic diversity and genetic structure of the shrimps and development of microsatellite markers and molecular MAS of shrimps.
Dissertation: Genetic diversity studies of
Litopenaeus vannamei and
Fenneropenaeus chinnesis populations and the molecular phylogeny of family Peuaeoidea.
4. As a master student in South China Botanical Garden, Chinese Academy of Sciences, advised by Prof. Mingyong Zhang, I investigated the physiological functions of
Oryza sativa glutathione synthetase (OsGS) in the different tissues induced by stresses.
Thesis: Stress inducible expression analysis of glutathione synthetase gene in rice (
Oryza sativa).
5. As an undergraduate student in Guangzhou University, I study on the technology for fermention of low suger preserved fruit by lactic acid bacteria.
Research Projects
1. National Science Foundation for Young Scientists of China (Grant No. 31001132, 2010-2013, RMB180, 000): The differences study of immune response in head kidney and peripheral blood of red-spotted grouper induced by Vibrio alginolyticus.
2. China Postdoctoral Science Foundation (Grant No. 200915863, 2009-2011, RMB30, 000): The study of antiviral role of shrimp lysozyme.
3. China Postdoctoral Science Foundation (Grant No. 200915863, 2012-2014, RMB50, 000): Development and application of EST-SSR markers by data mining in Pacific white shrimp Litopenaeus vannamei.
4. The
Jiangsu
University
Senior Professional Science Foundation (
Grant No.10JDG075, 2010-2015 (RMB 100,000).
5. Foundation of marine resources and sustainable utilization key laboratory of
Chinese
Academy
of Sciences (RMB 30,000.00 No.200921, FY 2009.9-2011.9)
Publications
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1）Wei-jun Mai, Hong-xiao Liu, Hui-qing Chen, Yang-jing Zhou, Yan Chen.RGNNV-induced cell cycle arrest at G1/S phase enhanced viral replication via p53-dependent pathway in GS cells. Virus Research, (2018)256, 142-152.SCI 3区，IF:2.867
2）Wei-jun Mai, Fang Huang, Hui-qing Chen, Yang-jing Zhou, Yan Chen. Nervous necrosis virus capsid protein exploits nucleolar phosphoprotein nuclephosmin (b23) function for viral replication. Virus Research, (2016)230, 1-6.SCI 3区，IF:2.867
3）Wei-jun Mai, Peng Liu, Huiqing Chen, Yajing Zhou.Cloning and immune characterization of the c-type lysozyme gene inred-spotted grouper,Epinephelus akaara. Fish and Shellfish Immunology, 36 (2014) 305e314SCI 1区, IF:3.521
4）Wei-jun Mai, Fang Huang.The role of EaIκB-α, a IκB-α homologue inEpinephelus akaara, involved in innate immune response.Biotechnology Letter, (2014) 36:1421–1429.SCI 3区，IF:1.853
5）Wei-jun Mai, Peng Liu. Characterization of tilapia p53 gene and its role in chemicals-induced apoptosis in tilapia. Biotechnology Letter, 2012.03. IF:1.853
6)Wei-jun Mai, Weina Wang. Acute acidic exposure induces p53-mediated Oxidative stress and DNA damage in the blood cells of tilapia (Oreochromis niloticus). Aquatic Toxicology IF:3.576. 2010, 100:271-281.
7)Wei-jun Mai, Chaoqun Hu, Weina Wang. In vitro activation of the antibacterial activity by virus-resistant shrimp (Marsupenaeus japonicus) recombinant interferon-like protein. Aquaculture IF:2.125, 2009, 288: 140-142；
8)Wei-jun Mai, Weina Wang. Protection of blue shrimp against white spot syndrome virus (WSSV) challenge by shrimp lysozyme. Fish and Shellfish immunity IF:3.161. 2010, 28:727-733.
9) Wei-jun Mai, Chaoqun Hu. cDNA cloning, expression and antibacterial activity of lysozyme C in the blue shrimp ( Litopenaeus stylirostris). Progress in natural science，2009, 19(7): 837-844.
10)Wei-jun Mai. Response to the letter on the shrimp intlP. Aquaculture. 2009, 298(1-2): 183.
11)Weijun Mai, Chaoqun Hu. Molecular cloning, characterization, expression and antibacterial analysis of a lysozyme homologue from Fenneropenaeus merguiensis. Molecular biology reports IF: 1.750, 2008, 26(2): 192-197.
12) Wei-Na Wang, Jun Zhou,Wei-jun Mai. Oxidative stress, DNA damage and antioxidant enzyme gene expression in the Pacific white shrimp, Litopenaeus vannamei when exposed to acute pH stress.COMP BIOCHEM PHYS C.(2009), doi:10.1016/j.cbpc.2009.06.010
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PATENT
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1. A high energy production and application of lysozyme sterilization. No.201210055012.5 First Inventor.
2. A high enzyme activity of human DNA polymerase delta purification method. No.201010556585.7
Third inventor.
3. Application of mulberry silkworm bioreactor preparation of DNA polymerase delta method. ZL 2010. 10556599.9 Six inventor.
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