Curriculum Vitae

    Hengchuan Xia, Ph.D.                                  

   Assistant professor of Institute of Life Sciences

   Jiangsu University 301 XueFu road Zhenjiang, Jiangsu, China, 212013

   Email: hchxia@ujs.edu.cn

   Phone: 0511-88791923

Research Interests:

 We are interested in the structure, activity, interaction and physiological function of biomolecules (such as proteins) involved in important cellular processes, such as protein trafficking,endosomal sorting and biosynthesis of metabolites. Our study will help improve the fundamental understanding of the mechanism and regulation of bio-processes, and may lead to discovery of noval ways for drug designs. We mainly employ X ray crystallography method to resolve the crystal structures of proteins and/or protein complexes, and study their biochemical and physiological functions via combined use of structure-based mutagenesis and gene knock-out and knock-in strategies.

Project 1, The silkworm MVB (multivesicular body) pathway and its involvement in the life cycle of BmNPV (Bombyx mori nuclearpolyhedrosis virus).  It is supported by the start-up fund for distinguished scholars of Jiangsu University (09JDG005), the natural science fund for colleges and universities in Jiangsu Province (10KJB180001), and the national natural science fund (31372259). We hope this study may further our knowledge about the interaction and gameplay between silkworm and BmNPV, providing insights into the infection,replication,viron assembly and release of BmNPV.

Project 2, The siderophore biosynthesis pathway in bacterium. We are focusing on the pyoverdine biosynthesis in Pseudomonas aeruginosa, and are supported by State Key Lab of Microbiological Engineering Research Fund (M2008-07) and the national natural science fund (31170692). We hope this work may illuminate the biosynthesis mechanism of pyoverdine, which may facilitate its application in clinical treatment of various diseases due to shortage or overload of iron ions and in environmental remediation caused by improper discharge of heavy metal ions.

We are also actively participating in other projects, such as those involving the roles of   N-acety-beta-D-glucosaminidase or amino acid transporter in the life cycle of BmNPV (31101673, 31301919). Another project is supported by the Ministry of Agriculture of China, which is focused on the development of high-throughput detection technique to identify transgenic rice samples...

Education

1993-1997, Qu Fu Normal University, General Biology, B.Sc.

1997-2004, Shanghai Institute of Biochemistry and Cellular Biology, Chinese Academy of Science, Biochemistry and Molecular Biology, Ph.D.

2004-2008, University of Michigan, U.S., Structural Biology, Cellular Biology, Visiting Scholar

Selected Publications:

1. Lü P#, Xia H#, Gao L, Pan Y, Wang Y, Cheng X, Lü H, Lin F,     Chen L, Yao Q, Liu X, Tang Q, Chen K. V-ATPase Is Involved in Silkworm Defense Response against Bombyx mori Nucleopolyhedrovirus. PLoS One. 2013 Jun 18;8(6):e64962. (# co-first author)

2. 夏恒传，张春霞，冯凡，袁弋，周阳，刘晓勇，朱克明，姚勤，陈克平。多囊体生物发生和蛋白质分拣：ESCRT、Vps4、Ubiquitination一个都不能少. 生物化学与生物物理进展，2013, 40(2):103-117

3. Hengchuan Xia, Chunxia Zhang, Fan Feng, Yi Yuan, Xiaoyong Liu, Keming Zhu, Qin Yao, Keping Chen. The multivesicular body pathway: A newly discovered battlefield for gameplay between virus and host. 2013, 7(11):913-920  , African Journal of Microbiology Research

4. Qin L#, Xia H#, Shi H, Zhou Y, Chen L, Yao Q, Liu X, Feng F, Yuan Y, Chen K.  Comparative proteomic analysis reveals that caspase-1 and serine protease may be involved in silkworm resistance to Bombyx mori nuclear polyhedrosis virus. J Proteomics. 2012 Jun 27;75(12):3630-8. doi: 10.1016/j.jprot.2012.04.015. (# co-first author)

5. Xia H, Zhang C, Feng F, Yuan Y, Zhou Y, Liu X, Zhu K, Yao Q, Chen K.  Molecular cloning, expression and characterization of a novel vacuolar protein sorting 4 gene in silkworm, Bombyx mori. Mol Biol Rep. 2012 Dec;39(12):10339-46

6. Xia H, Wu C, Xu Q, Shi J, Feng F, Chen K, Yao Q, Wang Y, Wang L. Molecular cloning and characterization of lactate dehydrogenase gene 1 in the silkworm, Bombyx mori. Mol Biol Rep. 2011 Mar;38(3):1853-60

7. Hengchuan Xia, Honggang Lv Keping Chen, Qin Yao, Peng Lv, Jun Li, Huiqing Chen and Lin Wang.Cloning and characterization of peptidylprolyl isomerase B in the silkworm, Bombyx mori. African Journal of Biotechnology. 2009,8(24):7148-7155

8. Pan Y, Xia H, Lü P, Chen K, Yao Q, Chen H, Gao L, He Y, Wang L. Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori.Acta Biochim Pol. 2009;56(4):671-7

9. Xiao J, Xia H, Zhou J, Azmi IF, Davies BA, Katzmann DJ, Xu Z. Structural basis of vta1 function in the multivesicular body sorting pathway. Dev Cell. 2008, 14(1): 37-49

10. Xiao J, Xia H, Yoshino-Koh K, Zhou J, Xu Z. Structural characterization of the ATPase reaction cycle of endosomal AAA protein Vps4. J Mol Biol. 2007, 374(3): 655-70

11. Xia HC, Li F, Li Z, Zhang ZC. Purification and characterization of Moschatin, a novel type I ribosome-inactivating protein from the mature seeds of pumpkin (Cucurbita moschata), and preparation of its immunotoxin against human melanoma cells. Cell Research, 2003, 13 (5): 369-74

12.  Xia HC, Hu WG, Yang XX, Li F, Zhang ZC. Preparation and primary application of monoclonal antibodies against a novel ribosome-inactivating protein Moschatin from pumpkin seeds.  Acta Biochim Biophys Sin (Shanghai). 2004, 36 (2): 105-10.