Curriculum Vitae

Xia Hengchuan

Associate professor  

Ph.D. in Biochemistry and Molecular biology

Tel: 0511-88791923

Email:hchxia@ujs.edu.cn

Location: Room F405, Lei Si Building, Jiangsu University

Biography

窗体顶端

1993-1997, Qu Fu Normal University, General Biology, B.Sc.
1997-2004, Shanghai Institute of Biochemistry and Cellular Biology, Chinese Academy of Science, Biochemistry and Molecular Biology, Ph.D.
2004-2008, University of Michigan, U.S., Structural Biology, Cellular Biology, Visiting Scholar

2008-today, School of Life Sciences, Jiangsu University, China.

Fields of research:

We are working in the field of insect biology and insect virology. Currently, we are interested in the exploration of the molecular mechanisms underlying the silkworm resistance to the infection of BmNPV.

Silkworm is an economically valuable insect and a model organism for insect research. Silkworms are usually susceptible to BmNPV infection, which can cause great losses in sericulture. Our leading researcher Professor Chen Keping has identified a silkworm variety with high resistance to BmNPV infection. During the past decades, we have performed a large-scale comparative genomic, transcriptomic and proteomic studies and have identified many genes and proteins possibly involved in the silkworm resistance to BmNPV infection. We are currently conducting further research on the molecular functions of these candidate genes and proteins in order to search for the most essential ones that determine the silkworm anti-virus traits. Our study may guide the efforts to generate novel silkworm varieties with improved resistance to BmNPV and other pathogens as well.

Among these candidates, we noticed that suppressors of cytokine signaling (SOCSs) may be important members involved in the silkworm resistance against BmNPV. SOCSs can negatively regulate the classic JAK-STAT-IFN and NF-kB signaling pathways, and play important roles in host innate immune responses against various pathogens. They are commonly induced by various kinds of viruses to help evade the host immune responses, while host cells can also compete with viruses for the control of SOCSs to mount varied levels of antiviral responses. Currently most of studies are focused on vertebrate SOCSs (especially mammalian SOCSs), and there are only a few reports on the physiological and pathological roles of invertebrate SOCSs including especially insects. We are working to identify all silkworm SOCS members and to investigate their roles in the infection of BmNPV and in the silkworm responses against BmNPV.

Projects:

1. The action mechanisms of silkworm proteins BmVps4 and BmVta1 in silkworm Multivesicular body (MVB) pathway and in the infection process of Bombyx mori nucleopolyherovirus (BmNPV). National Natural Science Foudation of China (31372259).

2. Effects of silkworm β - n-acetylglucosaminidase 2 on glycosylation of Bombyx mori nucleopolyherovirus (BmNPV). National Natural Science Foudation of China (31101673)

3. The silkworm amino acid transporters and their anti- Bombyx mori nucleopolyherovirus (BmNPV) functions. National Natural Science Foudation of China (31301919).

4. The silkworm Multivesicular body (MVB) pathway and its role in the infection process of Bombyx mori nucleopolyherovirus (BmNPV). Natural Science Foundation for Universities of Jiangsu Province (10KJB180001).

5. The structures and functions of important proteins in the bio- synthesis of siderophore pyoverdine in periplasmic space of Pseudomonas aeruginosa. National Natural Science Foudation of China (31170692).

6. The structures and functions of proteins involved in the bio- synthesis of siderophores of Pseudomonas aeruginosa. National Key lab of Microbiotechnology of China (M2008-07)

Main Publications:

1. Wang Q, Cui M, Zhang C, Xia A, Wang Q, Liu X, Chen K, Xia H. Cloning of suppressor of cytokine signaling 7 from silkworm (Bombyx mori) and its response to the infection of Bombyx mori nucleopolyhedrovirus. Arch Insect Biochem Physiol. 2024 Jan;115(1):e22065. doi: 10.1002/arch.22065.

2. M Cui, Q Wang, C Zhang, A Xia, Q Wang, X Liu, K Chen, H Xia. Cloning and subcellular localization of silkworm suppressor of cytokine signaling 6 and its expressional changes in response to the infection of Bombyx mori nucleopolyhedrovirus. Invertebrate Survival Journal. 2024,21:9-24

3. Xin Zhang, Qian Wang, Miao Cui, Cong Zhang, Yue Wu, Hengchuan Xia. Molecular identification of suppressor of cytokine signaling 2 (SOCS2its response to BmNPV in Bombyx mori (Lepidoptera: Bombycidae). Journal of Asia-Pacific Entomology. 2024, 27(2), 102226. https://doi.org/10.1016/j.aspen.2024.102226

4. Zhang, X.; H. Xia; Q. Wang; M. Cui; C. Zhang; Q. Wang; X. Liu; K. Chen. SOCSs: important regulators of host cell susceptibility or resistance to viral infection. Z Naturforsch C J Biosci. 2023 May 29;78(9-10):327-335. doi: 10.1515/znc-2023-0024.  

5. Xiao, R.; Y. Yuan; H. Xia; Q. Ge; L. Chen; F. Zhu; J. Xu; X. Wang; Y. Fan; Q. Wang; Y. Yang; K. Chen. Comparative transcriptome and proteome reveal synergistic functions of differentially expressed genes and proteins implicated in an over-dominant silkworm heterosis of increased silk yield. Insect Mol Biol. 2022, 31, 5, 551-567.

6. Wang, Q.; Z. Sun; S. Ma; X. Liu; H. Xia; K. Chen. Molecular mechanism and potential application of bacterial infection in the silkworm, Bombyx mori. Dev Comp Immunol. 2022, 131, 104381.

7. Wang, Q.; J. Wang; M. Ren; S. Ma; X. Liu; K. Chen; H. Xia. Peptidoglycan recognition protein-S1 acts as a receptor to activate AMP expression through the IMD pathway in the silkworm Bombyx mori. Dev Comp Immunol. 2021, 115, 103903.

8. Wang, Q.; M. Ren; X. Liu; H. Xia; K. Chen. Identification and characterization of novel short-type BmPGRP-S4 from the silkworm, Bombyx mori, involved in innate immunity. Z Naturforsch C J Biosci. 2020, 75, 1-2, 13-21.

9. Xia, H.; L. Chen; D. Shao; X. Liu; Q. Wang; F. Zhu; Z. Guo; L. Gao; K. Chen. Vacuolar protein sorting 4 is required for silkworm metamorphosis. Insect Mol Biol. 2019, 28, 5, 728-738.

10. Wang, Q.; M. Ren; X. Liu; H. Xia; K. Chen. Peptidoglycan recognition proteins in insect immunity. Mol Immunol. 2019, 106, 69-76.

11. Yuan, Z.; F. Gao; G. Bai; H. Xia; L. Gu; S. Xu. Crystal structure of PvdO from Pseudomonas aeruginosa. Biochem Biophys Res Commun. 2017, 484, 1, 195-201.

12. Xia, H.; D. Shao; X. Liu; Q. Wang; Y. Zhou; K. Chen. Identification and Characterization of BmVta1, a Bombyx mori (Lepidoptera: Bombycidae) Homologue for Vta1 That is Up-Regulated in Development. J Insect Sci. 2017, 17, 3, 1-7.

13. Tang, Q.; P. Wu; Z. Hu; Y. Yang; L. Qiu; H. Liu; S. Zhu; Z. Guo; H. Xia; K. Chen; G. Li. Evidence for the role of BmNPV Bm65 protein in the repair of ultraviolet-induced DNA damage. J Invertebr Pathol. 2017, 149, 82-86.

14. 邵丹丹，夏恒传，陈波，余毛毛，陈克平. 多功能分子机器ESCRT的研究进展. 江苏大学学报（医学版）. 2016, 26, 4, 360-366.

15. Han, Y.; H. Xia; Q. Tang; P. Lü; S. Ma; Y. Yang; D. Shao; Q. Ma; K. Chen. The Bombyx mori nucleopolyhedrovirus Bm111 affects virulence but not virus replication. Curr Microbiol. 2014, 69, 1, 56-62.

16. 夏恒传，张春霞，冯凡，袁弋，周阳，刘晓勇，朱克明，姚勤，陈克平. 多囊体生物发生和蛋白质分拣 - ESCRT、Vps4、Ubiquitination一个都不能少. 生物化学与生物物理进展. 2013, 40, 2, 103-117.

17. Xia, H.; C. Zhang; F. Feng; Y. Yuan; X. Liu; K. Zhu; Q. Yao; K. Chen. The multivesicular body pathway: A newly discovered battlefield for gamplay between virus and host. African Journal of Microbiology Research. 2013, 7, 11, 913-920.

18. Lü, P.; H. Xia; L. Gao; Y. Pan; Y. Wang; X. Cheng; H. Lü; F. Lin; L. Chen; Q. Yao; X. Liu; Q. Tang; K. Chen. V-ATPase Is Involved in Silkworm Defense Response against Bombyx mori Nucleopolyhedrovirus. PLoS One. 2013, 8, 6, e64962.

19. Xia, H.; C. Zhang; F. Feng; Y. Yuan; Y. Zhou; X. Liu; K. Zhu; Q. Yao; K. Chen. Molecular cloning, expression and characterization of a novel vacuolar protein sorting 4 gene in silkworm, Bombyx mori. Mol Biol Rep. 2012, 39, 12, 10339-46.

20. Qin, L.; H. Xia; H. Shi; Y. Zhou; L. Chen; Q. Yao; X. Liu; F. Feng; Y. Yuan; K. Chen. Comparative proteomic analysis reveals that caspase-1 and serine protease may be involved in silkworm resistance to Bombyx mori nuclear polyhedrosis virus. J Proteomics. 2012, 75, 12, 3630-8.

21. Xia, H.; C. Wu; Q. Xu; J. Shi; F. Feng; K. Chen; Q. Yao; Y. Wang; L. Wang. Molecular cloning and characterization of lactate dehydrogenase gene 1 in the silkworm, Bombyx mori. Mol Biol Rep. 2011, 38, 3, 1853-60.

22. Liu, X.; K. Chen; Q. Yao; H. Xia. Proteomic analysis of differentially expressed proteins involved in BmNPV resistance in the fat body of silkworm, Bombyx mori. Z Naturforsch C. 2010, 65, 11-12, 713-8.

23. Pan, Y.; H. Xia; P. Lu; K. Chen; Q. Yao; H. Chen; L. Gao; Y. He; L. Wang. Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori. Acta Biochim Pol. 2009, 56, 4, 671-7.

24. Xiao, J.; H. Xia; J. Zhou; I.F. Azmi; B.A. Davies; D.J. Katzmann; Z. Xu. Structural basis of Vta1 function in the multivesicular body sorting pathway. Dev Cell. 2008, 14, 1, 37-49.

25. Xiao, J.; H. Xia; K. Yoshino-Koh; J. Zhou; Z. Xu. Structural characterization of the ATPase reaction cycle of endosomal AAA protein Vps4. J Mol Biol. 2007, 374, 3, 655-70.

26. Xia, H.C.; W.G. Hu; X.X. Yang; F. Li; Z.C. Zhang. Preparation and primary application of monoclonal antibodies against a novel ribosome-inactivating protein Moschatin from pumpkin seeds. Acta Biochim Biophys Sin (Shanghai). 2004, 36, 2, 105-10.

27. Xia, H.C.; F. Li; Z. Li; Z.C. Zhang. Purification and characterization of Moschatin, a novel type I ribosome-inactivating protein from the mature seeds of pumpkin (Cucurbita moschata), and preparation of its immunotoxin against human melanoma cells. Cell Res. 2003, 13, 5, 369-74.